Brn3b (Brain-Specific Homeobox/POU Domain Protein 3B) or POU4F2 (POU Class 4 Homeobox 2) is a DNA-binding transcriptional regulator and coregulator that recognizes and binds to the consensus octamer binding site 5-AT[A/T]A[T/A]T[A/T]A-3 in promoter of target genes. Brn3b plays a fundamental role in the gene regulatory network essential for retinal ganglion cell (RGC) differentiation. It cooperates with the transcription factor ISL1 to achieve RGC fate specification in the developing retina. And plays a role in RGC axon formation and guidance by regulating gene expression of specific target genes. Brn3b plays a role in TNFSF11-mediated terminal osteoclast differentiation. It binds chromatin at promoter region of target genes. It acts either as a transcriptional activator or repressor. It plays a role in the regulation of breast cancer cell growth by promoting transcription activation as well as repression of specific target genes. It is involved in tumor breast progression and invasion. Brn3b also plays a role either as a transcriptional coactivator or corepressor. Anti-Brn3b Antibody is useful for researchers interested in breast cancer research, transcription factor activity, and DNA binding.
Anti-Brn3b antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to a near N-terminal portion of mouse Pou4f2 conjugated to Keyhole Limpet Hemocyanin (KLH).
来源宿主
Goat
反应性
Mus musculus (Mouse); Rattus (Rat)
保存建议
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
