HSP70 is a class of highly conserved stress proteins. HSP70 is comprised of multiple members，all of which bind ATP in vitro，but are localized within different intracellular compartments. These include: a） the constitutive Hsc70 （or cognate） present within the cytosol/nucleus； b） the highly stress-inducible HSP70 present within the cytosol/ nucleus/nucleolus； c） the constitutive glucose regulated 78 kDa （or BiP） protein present within the lumen of the endoplasmic reticulum； and d） the glucose regulated 75 kDa protein present within the mitochondria. HSP70 is typically not expressed in the cell under normal growth conditions，but is expressed at high levels in the cell experiencing stress. Therefore，detection of HSP70 using an antibody specific for the inducible form is quite useful in ascertaining whether a stress response has occurred in the cell.
应用类型
The antibody can be used in Western Blot analysis （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. Recombinant human HSP70 （Cat.# 4859-25） can be used as a positive control
免疫原
E. coli-expressed recombinant human HSP70
来源宿主
兔
反应性
经检测验证，可与人种属来源的HSP70蛋白发生免疫反应
保存建议
提供的100 ug HSP70抗体产品为无色溶液其浓度为0.5 mg/ml溶解于包含有30%甘油， 0.5% BSA， 5 mM EDTA和0.01%硫柳汞的PBS （pH 7.2）缓冲液中.请将该HSP70抗体产品置于-20 °C冷冻保存，建议分装使用以避免反复冻融.
