Wnt-1 is a secreted protein that signals through the Frizzled family of cell surface receptors and is required for normal embryonic development. Wnt-1 activation induces a complex signaling cascade that ultimately leads to the increased expression of over fifty genes. An important component of Wnt-1 signaling is the stabilization，and resulting accumulation，of the intracellular signaling protein，beta-catenine. Wnt signaling induces and maintains the transformed phenotype and，in certain embryonic cell lines，supports self renewal in the absence of significant differentiation. Elevated levels of Wnt proteins are associated with tumorigenesis and are present in numerous human breast cancers. Mature human Wnt-1 is a glycosylated protein containing 343 amino acid residues.
应用类型
Western blot analysis （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. Recombinant human Wnt-1 （Cat.# 4754-10） can be used as a positive control.
免疫原
E. coli expressed human Wnt-1
来源宿主
兔
反应性
经检测验证，可与人种属来源的Wnt-1蛋白发生免疫反应.
保存建议
提供的Wnt-1抗体产品为100 ug 无色溶液，其浓度为0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS （pH 7.2）缓冲液中.请将该Wnt-1抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
