ApoE belongs to a group of proteins that bind reversibly with lipoprotein and play an important role in lipid metabolism. In addition to facilitating solublization of lipids，these proteins help to maintain the structural integrity of lipoproteins，serve as ligands for lipoprotein receptors，and regulate the activity of enzymes involved in lipid metabolism. Significant quantities of ApoE are produced in liver and brain and to some extent in almost every organ. ApoE is an important constituent of all plasma lipoproteins. It’s interaction with specific ApoE receptor enables uptake of chylomicron remnants by liver cells，which is an essential step during normal lipid metabolism. It also binds with the LDL receptor （apo B/E）. Defects in ApoE are a cause of hyperlipoproteinemia type III. ApoE exists in three major isoforms； E2，E3，and E4，which differ from one another by a single amino-acid substitution. Individuals heterozygous for the ApoE4 allele are at higher risk of late-onset Alzheimer’s disease.
应用类型
Western blot analysis （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. Recombinant human ApoE4 （Cat.# 4699-50） can be used as a positive control.
免疫原
Recombinant human ApoE4
来源宿主
兔
反应性
经检测验证，可与人种属来源的ApoE4蛋白发生免疫反应.
保存建议
提供的ApoE4抗体产品为100 ug 无色溶液，其浓度为0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞nn的PBS （pH 7.2）缓冲液中.请将该ApoE4抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
