The mammalian Matrix metalloproteinases （MMPs） degrade extracellular matrix in physiological and pathological processes. After cleavage of a single peptide domain of about 20 amino acids，the MMPs are secreted in latent forms. Upon activation，the N-terminal propeptide domain is cleaved to generate the active forms of MMP. MMP-9 （92 kDa type IV collagenase，Gelatinase-B） contains the basic structure of propeptide，catalytic，and hemopexin domains. It is an important proteinase in tissue remodeling.
应用类型
Western blot analysis （0.5-4 ug/ml）， immunoprecipitation （4-8 ug/ml）， and Immunohistochemistry （20-40 ug/ml）. However， the optimal conditions should be determined individually.
免疫原
E. coli-expressed active rat 92-kDa type IV collagenase
来源宿主
兔
反应性
经检测验证，可与大鼠种属来源的MMP-9蛋白发生免疫反应.
保存建议
提供的MMP-9抗体产品为100 ug 无色溶液，其浓度为0.5 mg/ml，溶解于包含有50%甘油， 1% BSA， 0.02%硫柳汞的PBS （pH 7.2）缓冲液中.请将该MMP-9抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
