Human immunodeficiency virus （HIV） and related virus require coreceptors，in addition to CD4，to infect target cells. Some G protein-coupled receptors including CCR5，CXCR4，CCR3，CCR2b and CCR8 in the chemokine receptor family，and four new human molecules GPR15，STRL33，GPR1 and V28 were recently identified as HIV coreceptors. Among them，CXCR4 （fusin，LESTR or HUMSTR） is a principal coreceptor for T-cell tropic strains of HIV-1 fusion and entry of human white blood cells. CXCR4 is also required for the infection by dual-tropic strains of HIV-1 and mediates CD-4 independent infection by HIV-2. The -chemokine SDF-1 is the ligand for CXCR4 and prevents infection by T-tropic HIV-1. CXCR4 associates with the surface CD4-gp120 complex before HIV enters target cells. CXCR4 messenger RNA levels correlated with HIV-1 permissiveness in diverse human cell types. Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells. The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV binding sites.
应用类型
Western blot analysis （0.5-4 ug/ml）， immunoprecipitation， and immunocytochemistry. However， the optimal conditions should be determined individually. A 43 kDa band should be detected. HeLa cell lysate can be used as a positive control.
免疫原
Synthetic peptide mapping to the N-terminus of human CXCR4
来源宿主
兔
反应性
经检测验证，可与人、小鼠种属来源的CXCR4蛋白发生免疫反应.
保存建议
提供的CXCR4抗体产品为100 ug 无色溶液，其浓度为0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞的PBS （pH 7.2）缓冲液中.请将该CXCR4抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
