The originally described IL-17 protein，now known as IL-17A，is a homodimer of two 136 amino acid chains，secreted by activated T-cells that act on stromal cells to induce production of proinflammatory and hematopoietic bioactive molecules. Today，IL-17 represents a family of structurally-related cytokines that share a highly conserved C-terminal region but differ from one another in their N-terminal regions and in their distinct biological roles. The six known members of this family，IL-17A through IL-17F，are secreted as homodimers. IL-17A exhibits cross-species bioactivity between human and murine cells.
应用类型
1） WB: Use 0.1-0.2 ug/ml. The detection limit for recombinant human IL-17A is 1.5-3.0 ng/lane， under either reducing or non-reducing conditions. 2） ELISA: Use 0.5 - 2.0 ug/ml （100 ul/well antibody solution） 3） Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIL-17A （50.0 ng/ml）， a concentration of 0.9-1.3 ug/ml of this antibody is required. 4） Immunohistochemistry: 0.25 ug/ml with an overnight incubation at 4°C.
