IL-7 is a hematopoietic growth factor which affects primarily early B and T cells. Produced by thymic stromal cells,spleen cells and keratinocytes,IL-7 can also co-stimulate the proliferation of mature T cells in combination with other factors such as Con A and IL-2. Human and murine IL-7 is cross-species reactive.
应用类型
1) WB: Use 0.1-0.2 ug/ml. The detection limit for recombinant human IL-7 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 2) ELISA: Use 0.5 - 2.0 ug/ml (100 ul/well antibody solution) 3) Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human IL-7 (1.0 ng/ml), a concentration of 0.04 - 0.06 ug/ml of this antibody is required.