Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 （BH3） domain is a potent death domain. BH3 domain containing pro-apoptotic proteins，including Bad，Bax，Bid，Bik，Hrk，Nip3，and Bim，form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bnip3L，Bnip3alpha，and Nix （for Nip3-like protein X）. Bnip3L/Bnip3alpha/Nix is a homolog of the E1B 19K/Bcl-2 binding and pro-apoptotic protein Bnip3. Overexpression of Bnip3L induces apoptosis. Bnip3L interacts with and overcomes suppresses by Bcl-2 and Bcl-xL. Bnip3L is localized in mitochondria. The messenger RNA of Bnip3L is ubiquitously expressed in human tissues. Bnip3L and Bnip3 form a new subfamily of the pro-apoptotic mitochondrial proteins.
应用类型
Immunohistochemistry: 2 ug/ml； Western Blot: 0.5 – 1 ug/ml； ELISA. However， the optimal conditions should be determined individually.
免疫原
A synthetic peptide corresponding to the amino acids 77 to 92 of human Bnip3L.
