Accumulation of the amyloid-beta peptide （Abeta） in the cerebral cortex is a critical event in the pathogenesis of Alzheimer’s disease. The beta-amyloid protein precursor （APP） is cleaved by beta-secretase，producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment （C99）. The C99 fragment serves as substrate for gamma-secretase to generate the 4 kDa amyloid-beta peptide （Abeta），which is deposited in the brains of all suffers of Alzheimer’s disease.
应用类型
Immunohistochemistry: 2 ug/ml； Western Blot: 1 – 2 ug/ml； ELISA. However， the optimal conditions should be determined individually.
免疫原
A peptide corresponding to the amino acids 737 to 751 of APP or 85 to 99 of the C99 fragment generated by b-secretase cleavage.
