Tyrosine (Tyr or Y) or 4-hydroxyphenylalanine is a hydrophilic amino acid. In plants and most microorganisms, tyrosine and α-ketoglutarate are produced via prephenate, when oxidatively decarboxylated with retention of the hydroxyl group to give p-hydroxyphenylpyruvate, which is transaminated using glutamate as the nitrogen source. Mammals synthesize tyrosine from the essential amino acid phenylalanine (phe), which is derived from food. The conversion of phe to tyr is catalyzed by the enzyme phenylalanine hydroxylase causing the addition of a hydroxyl group. In its phosphorylated form, tyrosine is called phosphotyrosine. Tyrosine phosphorylation is one of the key steps in signal transduction and regulation of enzymatic activity. Protein kinases can tag on the hydroxyl group end with a phosphate group to some tyrosine residues causing a phosphorylated form. Tyrosine residues may also be modified by the addition of a sulfate group, a process known as tyrosine sulfation.
应用类型
ELISA,Western Blot,
免疫原
This monoclonal antibody was produced after repeated immunizations of balb/c mice with phosphotyrosine conjugated KLH.
来源宿主
Mouse
反应性
Phosphotyrosine
保存建议
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
