DYKDDDDK Tag antibody detects over-expressed proteins containing the D epitope tag fused to either the amino- or carboxy-terminus of targeted proteins. Epitope tags are usually for the labeling and detection of proteins using immunoblotting，immunoprecipitation and immunostaining techniques. Due to their small size，they are unlikely to affect the tagged protein’s biochemical properties. The DYKDDDK epitope tag is widely used to detect expression of recombinant proteins in bacteria，yeast，insect and mammalian systems
应用类型
The antibody can be used for Western blot analysis （0.5-4 ug/ml）. However， the optimal conditions should be determined individually.
免疫原
A synthetic D- tag peptide DYKDDDDK conjugated to KLH.
来源宿主
兔
反应性
经检测验证，可与D-tag标签及D-tag融合蛋白种属来源的D-Tag标签蛋白发生免疫反应
保存建议
提供的100 ug D-Tag抗体产品为无色溶液其浓度为0.5mg/ml溶解于包含有30%甘油， 0.5% BSA， 5 mM EDTA和0.01%硫柳汞的PBS （pH 7.2）缓冲液中.请将该D-Tag抗体产品置于-20 °C冷冻保存，建议分装使用以避免反复冻融.
