The T7-tag is an 11 amino acid sequence encoded in the leader sequence of T7 bacteriophage gene10. T7 is used in many expression vectors including the pET system. Hence,the anti-T7-tag antibody is useful for studying expression of recombinant T7-tagged proteins.
应用类型
The antibody can be used for Western blot analysis (0.5-4 ug/ml). However, the optimal conditions should be determined individually.
免疫原
A synthetic T7-Tag peptide MASMTGGQQMG conjugated to KLH.
来源宿主
兔
反应性
经检测验证,可与T7-tag标签及T7-tag融合蛋白种属来源的T7标签蛋白发生免疫反应
保存建议
提供的100 ug T7 Epitope -Tag抗体产品为无色溶液其浓度为0.5mg/ml溶解于包含有30%甘油, 0.5% BSA, 5 mM EDTA和0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该T7 Epitope -Tag抗体产品置于-20 °C冷冻保存,建议分装使用以避免反复冻融.