- 中文名称
Transforming Growth Factor Alpha (TGFa) BioAssay ELISA Kit (Rabbit)
- 英文名字
- Transforming Growth Factor Alpha (TGFa) BioAssay ELISA Kit (Rabbit)
- 供应商
- USBiological
- 产品货号
- 396810
- 产品报价
- ￥1/96Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Transforming growth factor alpha(TGF-alpha) is upregulated in some Rabbit cancers. It is produced in macrophages, brain cells, and keratinocytes, and induces epithelial development. It is closely related to EGF, and can also bind to the EGF receptor with similar effects. TGFalpha stimulates neural cell proliferation in the adult injured brain. Transforming growth factor alpha gene(TGFA) maps to Rabbit chromosome 2 close to the breakpoint of the t(2;8) variant translocation in Burkitt lymphoma. Synthetic TGF-alpha was as active as murine epidermal growth factor in binding to the epidermal growth factor receptor and in stimulation of anchorage-dependent and of anchorage-independent growth of normal indicator cells in culture. Synthetic TGF-alpha stimulated plasminogen activator production in A 431 and HeLa cells; the stimulation was similar to that induced by epidermal growth factor. Furthermore, synthetic rabbit TGF-alpha showed similar immunoreactivity when compared with Rabbit TGF-alpha. Thus, the 50aa TGF-alpha is likely to be the bioactive principle produced and secreted by tumor cell lines.Sample Type:Cell culture supernates, serum, human milk, plasma (heparin or EDTA).Intended Use:Sandwich High Sensitivity BioAssay ELISA kit for Quantitative Detection of Rabbit TGF alpha..Sensitivity:<1pg/mlRange:15.6pg/ml-1000pg/mlSpecificity:Recognizes natural and recombinant Rabbit TGF alpha. There is cross-reactivity with TGF beta 2, TGF beta 3, TGF beta 5 (<1%).Test Principle:This Rabbit TGF Alpha BioAssay ELISA Kit was based on standard sandwich enzyme-linked immune- sorbent assay technology. A monoclonal antibody from mouse specific for Rabbit TGF Alpha has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli,V40-A89) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Rabbit TGF Alpha is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rabbit TGF Alpha amount of sample captured in plate.Kit Components:96-well plate precoated with anti-Rabbit TGF antibody, 1x plateLyophilized recombinant Rabbit TGF standard, 2x10ng/tubeBiotinylated anti-Rabbit TGF antibody, 1x130ul (dilution 1:100)Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100)Sample diluent buffer, 1x30mlAntibody diluent buffer, 1x12mlABC diluent buffer, 1x12mlTMB color developing agent, 1x10mlTMB stop solution, 1x10mlPBS washing buffer Powder for 1000ml, 1x bufferStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Transforming Growth Factor Alpha (TGFa) BioAssay ELISA Kit (Rabbit)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Transforming Growth Factor Alpha (TGFa) BioAssay ELISA Kit (Rabbit)产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
-
- 注意
-
该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
-
