Autophagy，the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR （Target of rapamycin） through phosphorylation of autophagy protein APG1. Another member of the autophagy family of proteins is APG7 which was identified in yeast as a ubiquitin-E1-like enzyme； this function is conserved in the mammalian homolog （4）. In mammalian cells，APG7 is essential for autophagy conjugation systems，autophagosome formation，starvation-induced bulk degradation of proteins and organelles. It has been suggested that caspase-8 may alter APG7 levels and thus the APG7 program of autophagic cell death.
应用类型
Western blotting （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. Other applications have not been determined.
免疫原
Synthetic peptide near the N-terminus of rat APG7
来源宿主
兔
反应性
经检测验证，可与人、小鼠种属来源的APG7/ATG7蛋白发生免疫反应.
保存建议
提供的APG7/ATG7抗体产品为100 ug 无色溶液，其浓度为0.5 mg/ml，溶解于包含有30%甘油， 0.5 mg/ml BSA和0.01%硫柳汞的PBS缓冲液中.请将该APG7/ATG7抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
