Clock is a basic helix-loop helix （bHLH） transcription factor that regulates circadian rhythms and sleep cycles. Clock contain a DNA-binding domain，a protein dimerization domain that heterodimerizes with BMAL1 and a glutamine-rich C terminus region. Clock dimerization with BMAL helps regulate nuclear translocation and binding of Clock to the E box elements stimulates circadian genes transcription.
应用类型
Western blotting （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. The antibody recognizes the 100 kDa and the ~62 kDa isoforms of CLOCK in samples from human， mouse and rat origins. Reactivity to other species has not been tested.
