Insulin-induced gene （InSig） localizes in the endoplasmic recticulum（ER） and is highly expressed in the liver and fibroblast cell lines. InSig1 and InSig2 play important roles in the regulation of cholesterol biosynthesis. Sterol induces InSig1 binding to the sterol-sensing domain of SREBP cleavage-activating protein （SCAP）. Both InSig1 and Insig2 prevent the export of SCAP from the ER and thus，inhibit cholesterol synthesis by preventing the proteolytic cleavage of SREBPs by the Golgi enzymes.
应用类型
Western blotting （0.5-4 ug/ml）. However， the optimal concentrations should be determined individually. The antibody recognizes ~30 kDa InSig-1 and its precursor （~50 kDa） in samples from human， mouse and rat origins. Reactivity to other species has not been tested.
免疫原
Synthetic peptide surrounding amino acid 248 of rat Insig1
来源宿主
兔
反应性
经检测验证，可与人、小鼠、大鼠、牛、仓鼠种属来源的Insig1蛋白发生免疫反应.
保存建议
提供的Insig1抗体产品为100 ug 无色溶液，其浓度为0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS缓冲液中.请将该Insig1抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
