- 中文名称
Heat Shock Protein 27 (HSP27) BioAssay ELISA Kit
- 英文名字
- Heat Shock Protein 27 (HSP27) BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- 385066
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- HSP27s belong to an abundant and ubiquitous family of small heat shock proteins (sHSP). It is an important HSP found in both normal human cells and cancer cells. The basic structure of most sHSPs is a homologous and highly conserved amino acid sequence, with an alpha-crystallin-domain at the C-terminal and the WD/EPF domain at the less conserved N-terminal. This N-terminal is essential for the development of high molecular oligomers (1, 2). HSP27-oligomers consist of stable dimers formed by as many as 8-40 HSP27 protein monomers (3). The oligomerization status is connected with the chaperone activity: aggregates of large oligomers have high chaperone activity, whereas dimers have no chaperone activity (4). HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. Other functions include chaperone activity (as mentioned above), thermotolerance in vivo, inhibition of apoptosis, and signal transduction. Specifically, in vitro, it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding of the HSP70 complex. HSP27 is also involved in the apoptotic signaling pathway because it interferes with the activation of cytochrome c/Apaf-1/dATP complex, thereby inhibiting the activation of procaspase-9. It is also hypothesized that HSP27 may serve some role in cross-bridge formation between actin and myosin (5). And finally, HSP27 is also thought to be involved in the process of cell differentiation. The up-regulation of HSP27 correlates with the rate of phosphorylation and with an increase of large oligomers. It is possible that HSP27 may play a crucial role in termination of growth (6).Sample Type:Cell lysates, Serum, TissueIntended Use:This ELISA BioAssay kit is used to quantitate HSP27 concentration in samples.Sensitivity:0.04ng/mlRange:0.2-13ng/mlSpecificity:Recognizes human HSP27.Test Principle:This ELISA BioAssay Kit is for the detection of free, unbound human Hsp27 in cell lysates, tissue extracts, and human serum samples. The latter matrix has significant quantities of Hsp27-complexing components which can obscure the detection of Hsp27 by this assay. Each BioAssay kit contains sufficient components to quantitate the Hsp27 concentration in up to 40 samples, tested in duplicate.Kit Components:ELISA Plate, 1x96wells5X Hsp27 Extraction Reagent, 1x10mlStandard, 2xvialsStandard and Sample Diluent, 1x50ml10X Wash Buffer Concentrate, 1x100mlBiotinylated Antibody Concentrate, 1x150ulBiotinylated Antibody Diluent, 1x13mlStreptavidin: HRP Concentrate, 1x150ulStreptavidin: HRP Diluent, 1x13mlTMB Substrate, 1x13mlStop Solution, 1x13mlStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Heat Shock Protein 27 (HSP27) BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Heat Shock Protein 27 (HSP27) BioAssay ELISA Kit产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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