Diacylglycerol acyltransferase is also known as DGAT1 is a microsomal enzyme. It is involved in the synthesis of fatty acids into triglycerides. DGAT utilizes diacylglycerol (DAG) and fatty acyl CoA as substrates to synthesize triacylglycerol. DGAT plays an important role in the metabolism of cellular diacylglycerol and is essential in the physiologic processes of higher eukaryotes that includes triacylglycerol metabolism such as intestinal fat absorption,lipoprotein assembly,adipose tissue formation,and lactation.
应用类型
Western blotting (0.5-4 ug/ml). However, the optimal conditions should be determined individually. The antibody recognizes ~60 kDa DGAT-1 of human, mouse, and rat origins. Reactivity to other species has not been tested.
免疫原
Synthetic peptide corresponding to residues surrounding amino acids 308 of mouse DGAT-1
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠种属来源的DGAT1蛋白发生免疫反应.
保存建议
提供的DGAT1抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA, 0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该DGAT1抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.