- 中文名称
Fibrillin-2 (FBN2) BioAssay ELISA Kit (Human)
- 英文名字
- Fibrillin-2 (FBN2) BioAssay ELISA Kit (Human)
- 供应商
- USBiological
- 产品货号
- 382844
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The Fibrillin-2 (FBN2) BioAssay ELISA Kit (Human) is a quantitative sandwich assay for the detection of FBN2 in human serum, plasma and other biological fluids.Detection Range:0.625-40ng/mlSensitivity:0.38ng/mlPrecision:Intra-Assay %CV: <10%Inter-Assay %CV: <10%Assay Principle:The microtiter plate provided in this kit has been pre-coated with an antibody specific to FBN2. Standards or samples are then added to the appropriate microtiter plate wells, followed by biotin-conjugated antibody specific to FBN2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the sandwich complex consisting of FBN2, biotin-conjugated antibody and HRP-Avidin, will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 2nm. The absorbance value is directly proportional to the concentration of FBN2. The concentration of FBN2 in the sample is determined by comparing the O.D. of the sample to the standard curve.Kit Components:382844A: Microtiter Strips, 8 wells x12 strips382844B: Standard, 2 vials382844C: Sample/Standard Diluent Buffer, 1x20ml382844D: Antibody-Biotin (100X), 1x120ul382844E: Antibody-Biotin Diluent, 1x14ml382844F: Avidin-HRP Conjugate (100X), 1x120ul382844G: Avidin-HRP Conjugate Diluent, 1x14ml382844H: TMB Substrate, 1x10ml382844J: Stop Solution, 1x10ml382844JK: Wash Buffer (25X), 1x30mlStorage and Stability:Unused kit is stable for 6 months after receipt. Store Microtiter Strips, Standard, and Antibody-Biotin at 4°C if kit will be used within 30 days; otherwise store at -20°C until expiration date. Store the rest of the components at 4°C. Protect HRP Conjugate and Substrate Reagent from light. For maximum recovery, centrifuge the original vials after thawing and prior to removing the cap.Assay Summary:1. Add 100ul standard or sample to each well and incubate for 90 minutes at 37°C 2. Aspirate the liquid.3. Add 100ul Antibody-Biotin working solution to each well and incubate for 60 minutes at 37°C4. Aspirate the liquid and wash plate 3 times.5. Add 100ul Avidin-HRP working solution to each well. Incubate for 30 minutes at 37°C6. Aspirate the liquid and wash plate 5 times.7. Add 90ul TMB substrate. Incubate for 15 minutes at 37°C8. Add 50ul Stop Solution. Read at 450nm immediately.9. Calculate results.
- 产品特点
- 针对Fibrillin-2 (FBN2) BioAssay ELISA Kit (Human)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Fibrillin-2 (FBN2) BioAssay ELISA Kit (Human)产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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