HSF 1 responds to cellular stress signals such as heat，heavy metals and oxidative reagents. It binds to heat shock response elements in the upstream region of heat shock gene and activates transcription. Upon activation，HSF 1 is trimerized and is localized in the nucleus where it binds the DNA.
应用类型
Western blotting （0.5-4 ug/ml）. However， the optimal concentrations should be determined individually. The antibody recognizes 100-120 kDa band， corresponding to glycosylated HSF 1 in samples from human and mouse origins. Reactivity to other species has not been tested.
免疫原
Synthetic peptide surrounding amino acid 515 of mouse HSF 1
来源宿主
兔
反应性
经检测验证，可与人、小鼠种属来源的HSF1蛋白发生免疫反应.
保存建议
提供的HSF1抗体产品为100 ug 无色溶液，其浓度为0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS缓冲液中.请将该HSF1抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
