Calnexin (CNX) is a 90kDa integral protein of the endoplasmic reticulum (ER). Calnexin belongs among chaperones,which are characterized by their main function of assisting protein folding and quality control,ensuring that only properly folded and assembled proteins proceed further along the secretary pathway. The function of Calnexin is to retain unfolded or unassembled N-linked glycoproteins in the endoplasmic reticulum.
应用类型
Western blotting (0.5-4 ug/ml). However, the optimal concentrations should be determined individually. The antibody recognizes ~90-100 kDa band, corresponding to glycosylated Calnexin in samples from human, mouse, rat, and dog origins. Reactivity to other species has not been tested.
免疫原
Synthetic peptide surrounding amino acid 580 of human Calnexin
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠、狗种属来源的Calnexin蛋白发生免疫反应.
保存建议
提供的Calnexin抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA和0.01%硫柳汞的PBS缓冲液中.请将该Calnexin抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.