Prohibitin is an evolutionarily conserved protein that has anti-proliferative activity. Prohibitin is a 30 kDa，post-synthetically modified protein found in the inner membrane of mitochondria and on the plasma membrane of B cells. It mediates B cell mutation. Prohibitin Controls cell cycle in both transcriptional and post-translational mechanisms. Prohibitin is a potential tumor suppressor. Prohibitin gene mutations are correlated with the breast cancer development and/or progression in over 80%0f the cell lines analyzed.
应用类型
Western blotting （0.5-4 ug/ml）， immunoprecipitation （10-20 ug/ml） and Immunofluorescence （5-10 ug/ml）. However， the optimal conditions should be determined individually. The antibody detects a 33 kDa protein， corresponding to the apparent molecular mass of Prohibitin on SDS-PAGE immunoblots， in samples from human， mouse and rat origins.
免疫原
Synthetic peptide surrounding amino acid 256 of human Prohibitin
来源宿主
兔
反应性
经检测验证，可与人、小鼠、大鼠种属来源的Prohibitin蛋白发生免疫反应.
保存建议
提供的Prohibitin抗体产品为100 ug 无色溶液，其浓度为0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞的PBS （pH 7.2）缓冲液中.请将该Prohibitin抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
