Xenopus Dickkopf （Dkk）-1 was initially discovered as a Wnt antagonist that plays an important role in head formation. By far，four members of Dkk have been identified in mammals. Each Dkk molecule contains two conserved cysteine-rich domains. Recent studies showed that the second Cys-rich domains of Dkk1 and Dkk2 inhibited Wnt-3a-activated signaling，whereas the first Cys-rich domains had no effects. In addition，the second Cys-rich domain of Dkk-2，but not that of Dkk-1，was able to activate the canonical pathway in the presence of LRP6，and this LRP-dependent signaling does not require Dvl.
应用类型
Western blotting （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. The antibody recognizes 57 kDa Dkk2 in samples from human， mouse and rat origins. A ~55 kDa and ~28 kDa Dkk2 fragments can also be detected in rat kidney tissue lysate. Reactivity to other species has not been determined.
免疫原
Synthetic peptide surrounding amino acid 241 of mouse Dkk2
来源宿主
兔
反应性
经检测验证，可与人、小鼠、大鼠种属来源的DKK2蛋白发生免疫反应.
保存建议
提供的DKK2抗体产品为100 ug 无色溶液，其浓度为0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS缓冲液中.请将该DKK2抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
