Xenopus Dickkopf （Dkk）-1 was initially discovered as a Wnt antagonist that plays an important role in head formation. By far，four members of Dkk have been identified in mammals. Each Dkk molecule contains two conserved cysteine-rich domains. Recent studies showed that the second Cys-rich domains of Dkk1 and Dkk2 inhibited Wnt-3a-activated signaling，whereas the first Cys-rich domains had no effects. In addition，the second Cys-rich domain of Dkk-2，but not that of Dkk-1，was able to activate the canonical pathway in the presence of LRP6，and this LRP-dependent signaling does not require Dvl.
应用类型
Western blotting （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. The antibody recognizes 30-35 kDa Dkk2 in samples from human， mouse and rat origins. A ~25 kDa band can also be detected， presumably to be the cleavage fragment of Dkk2.
免疫原
Synthetic peptide surrounding amino acid 241 of mouse Dkk2 （
来源宿主
兔
反应性
经检测验证，可与人、小鼠、大鼠种属来源的DKK2蛋白发生免疫反应.
保存建议
提供的DKK2抗体产品为100 ug 无色溶液，其浓度为0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS缓冲液中.请将该DKK2抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
