T-2 toxin is mycotoxin produced by several Fusarium species. Food crops as wheat，barely，corn，and their products are mainly contaminated. T-2 toxin is a threat for human health and animal husbandry. T-2 toxin primarily affects the blood，liver，kidneys，pancreas，muscles and function of the lymphocyte. The general symptoms of T-2 toxin poisoning are anorexia，vomiting，diarrhea，growth retardation，reproduction and neurological dysfunction. This BioAssay kit is a detection product developed based on ELISA technology，with operation time as short as 50 minutes.Sample Type：Feed 7.5ppbIntended Use：This BioAssay kit allows for the in vitro quantitative determination of T-2 toxin concentrations in feed.Sensitivity：0.15ppbRange：0.15-4.05ppbPrecision：Intra-assay： CV%<10%Inter-assay： CV%<10%Test Principle：This BioAssay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this BioAssay kit has been pre-coated with T-2 toxin antigen. Standards or samples are added to the appropriate microtiter plate wells with T-2 toxin specific antibody and Horseradish Peroxidase （HRP） conjugated anti-antibody. The competitive inhibition reaction is launched between pre-coated T-2 toxin and T-2 toxin in standards or samples with the T-2 toxin special antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of T-2 toxin in the standards or samples. The color development is stopped and the intensity of the color is measured.Kit Components：Assay plate，96T/48TStandard （96T），5x1mlStandard （48T），5x0.5mlHRP-conjugate （96T），1x7mlHRP-conjugate （48T），1x3.5mlAntibody （96T），1x7mlAntibody （48T），1x1x3.5mlTMB Substrate （96T），1x12mlTMB Substrate （48T），1x6mlStop Solution （96T），1x10mlStop Solution，（48T），1x5mlSample Diluent （2x） （96T），1x20mlSample DIluent （2x） （48T），1x10mlWash Buffer （10X） （96T），1x50mlWash Buffer （10X） （48T），1x25mlAdhesive Strip （96T/48T），4x stripsInstruction Manual （96T/48T），1x manualStorage and Stability：Store powder at 4°C liquid at -20°C. Store other components at 4°C.Stable for at least 6 months For maximum recovery of product，centrifuge the original vial after thawing and prior to removing the cap.
