Sample Type:Tissue (Chicken,Duck meat,porcine,prawn),feed,urineIntended Use:This BioAssay kit allows for the in vitro quantitative determination of Diethylsilbestrol concentrations in urine,tissue (porcine,chicken,fish,shrimp).Sensitivity:0.1ppbRange:0.1-2.7ppbPrecision:Intra-assay: CV%<10%Inter-assay: CV%<10%Test Principle:This BioAssay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this BioAssay kit has been pre-coated with Diethylsilbestrol. Standards or samples are added to the appropriate microtiter plate wells with Diethylsilbestrol specific antibody and Horseradish Peroxidase (HRP) conjugated anti-antibody. The competitive inhibition reaction is launched between pre-coated Diethylsilbe strol and Diethylsilbestrol in standards or samples with the Diethylsilbestrol special antibody. A substrate solution is added to the wells and the color develo ps in opposite to the amount of Diethylsilbestrol in the standards or samples. The color development is stopped and the intensity of the color is measuredKit Components:Assay plate,96T/48TStandard (96T),5x0.3mlStandard (48T),5x0.15mlHRP-conjugate (11X) (96T),1x0.7mlHRP-conjugate (11X) (48T),1x0.35mlAntibody (96T),1x7mlAntibody (48T),1x1x3.5mlSubstrate A (96T),1x7mlSubstrate A (48T),1x3.5mlSubstrate B (96T),1x7mlSubstrate B (48T),1x3.5mlStop Solution (96T),1x14mlStop Solution,(48T),1x7mlRedissolving Solution (10X),1x10mlRedissolving Solution (10X),1x5mlWash Buffer (20X) (96T),1x30mlWash Buffer (20X) (48T),1x15mlAdhesive Strip (96T/48T),4x stripsInstruction Manual (96T/48T),1x manualStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C.Stable for at least 6 months For maximum recovery of product,centrifuge the original vial after thawing and prior to removing the cap.