XLF （also called Cernunnos） interacts with the XRCC4-DNA ligase IV complex to promote DNA non-homologous end-joining. It directly interacts with the XRCC4-Ligase IV complex and siRNA-mediated downregulation of XLF in human cell lines leads to radio-sensitivity and impaired DNA non-homologous end-joining. XLF is homologous to the yeast non-homologous end-joining factor Nej1.
应用类型
Western blotting （1:500 – 1:2500） and Immunoprecipitation. However， the optimal concentrations should be determined individually. The antibody recognizes the XLF of human origin. Reactivity to other species has not been tested.
免疫原
Synthetic peptide of human XLF
来源宿主
兔
反应性
经检测验证，可与人种属来源的XLF蛋白发生免疫反应.
保存建议
提供的XLF抗体产品为100 ul 无色溶液，其浓度为N/A，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS缓冲液中.请将该XLF抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
