DNA methylation can help regulate transcriptional silencing via repressive transcription complexes，which include methyl-CpG-binding domain protein and Histone deacetylases （HDACs） DNMT1. This core enzyme for mammalian DNA methylation can also establish a repressive transcription complex consisting of DNMT1，HDAC2 and DMAP1. DMAP1 can interact directly with the transcriptional corepressor TSG101. The DNMT1-DMAP1 exists throughout the S-phase； HDAC2 joind DNMT1 and DMAP1 only during Late S phase. This provides a regulated means to deacetylate heterochromatin following replication
应用类型
Western blotting （0.5-4 ug/ml）. However， the optimal concentrations should be determined individually. Jurkat cell lysate （Cat.# 2401-100） and mouse small intestine tissue lysate （Cat.2601-100） can be used as positive controls. The antibody recognizes the DMAP1 of human， mouse and rat origins. Reactivity to other species has not been tested.
免疫原
Synthetic peptide surrounding amino acid 438 of mouse DMAP1
来源宿主
兔
反应性
经检测验证，可与人、小鼠、大鼠种属来源的DMAP1蛋白发生免疫反应.
保存建议
提供的DMAP1抗体产品为100 ug 无色溶液，其浓度为0.5 mg/ml，溶解于包含有30%甘油， 1% BSA和0.02%硫柳汞的PBS缓冲液中.请将该DMAP1抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
