Sample Type：Serum，plasma，tissue homogenates and other biological fluids.Intended Use：Sandwich ELISA，Double AntigenSensitivity：<0.938ng/mlRange：1.563-100ng/mlSpecificity：MonkeyTest Principle：This BioAssay kit was based on sandwich enzyme-linked immune-sorbent assay technology. GAD was pre-coated onto 96-well plates. And the Biotin-labeled GAD was used as detection antigen. The standards，test samples and Biotin-labeled GAD were added to the wells subsequently，and wash with wash buffer. HRP-Streptavidin Conjugate was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the GAD-Ab amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader，and then the concentration of GAD-Ab can be calculated.Kit Components：Micro ELISA Plate （Dismountable），8x12Lyophilized Standard，2xvialSample/Standard dilution buffer，20mlBiotin-detection antibody （Concentrated），120ulAntibody dilution buffer，10mlHRP-Streptavidin Conjugate （SABC），120ulSABC dilution buffer，10mlTMB substrate，10mlStop solution，10mlWash buffer （25X），30mlPlate Sealer，5xpiecesProduct Description，1xcopyStorage and Stability：Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product，centrifuge the original vial after thawing and prior to removing the cap.
