Sample Type：Serum，plasma，tissue homogenates and other biological fluids.Intended Use：Sandwich ELISA，Double AntigenSensitivity：<0.938ng/mlRange：1.563-100ng/mlSpecificity：HumanTest Principle：This BioAssay kit was based on sandwich enzyme-linked immune-sorbent assay technology. thrombin III was pre-coated onto 96-well plates. And the Biotin-labeled thrombin III was used as detection antigen. The standards，test samples and Biotin-labeled thrombin III were added to the wells subsequently，and wash with wash buffer. HRP-Streptavidin Conjugate was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the thrombin III antibody amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader，and then the concentration of anti-thrombin III antibody can be calculated.Kit Components：Micro ELISA Plate （Dismountable），8x12Lyophilized Standard，2xvialSample/Standard dilution buffer，20mlBiotin-detection antibody （Concentrated），120ulAntibody dilution buffer，10mlHRP-Streptavidin Conjugate （SABC），120ulSABC dilution buffer，10mlTMB substrate，10mlStop solution，10mlWash buffer （25X），30mlPlate Sealer，5xpiecesProduct Description，1xcopyStorage and Stability：Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product，centrifuge the original vial after thawing and prior to removing the cap.
产品特点
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保存建议
建议收到Anti-Thrombin III BioAssay ELISA Kit （Human）产品后将其置于4°C至-20°C保存。
