Sample Type：Serum，plasma，tissue homogenates and other biological fluids.Intended Use：Competitive ELISA，Coated with AntigenSensitivity：<3.75pg/mlRange：6.25-400pg/mlSpecificity：RatTest Principle：This BioAssay ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Ang1-9. During the reaction，Ang1-9 in the sample or standard competes with a fixed amount of Ang1-9 on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to Ang1-9. Excess conjugate and unbound sample or standard are washed from the plate，and HRP-Streptavidin （SABC） is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Ang1-9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.Kit Components：Micro ELISA Plate （Dismountable），8x12Lyophilized Standard，2xvialSample/Standard dilution buffer，20mlBiotin-detection antibody （Concentrated），60ulAntibody dilution buffer，10mlHRP-Streptavidin Conjugate （SABC），120ulSABC dilution buffer，10mlTMB substrate，10mlStop solution，10mlWash buffer （25X），30mlPlate Sealer，5xpiecesProduct Description，1xcopyStorage and Stability：Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product，centrifuge the original vial after thawing and prior to removing the cap.
