The neuronal calcium sensor (NCS) protein binds to calcium through EF-hand motifs,are expressed in neurons. They are implicated in the regulation of neurotransmitters released at the neuromuscular junctions. NCS-1 is widely distributed in the brain,spinal cord and dorsal root ganglia. It has been shown to regulate neurosecreation in a calcium-dependent manner,potentiates nitric oxide synthase activity. NCS-1 has been reserved to indicate the protein observed in humans and mammals.
应用类型
Western blotting (0.5-4 ug/ml). However, the optimal concentrations should be determined individually. Rat kidney tissue lysate can be used as a positive control. The antibody recognizes ~24 kDa NCS-1 from samples of human and rat origins. Reactivity to other species has not been determined.
免疫原
Synthetic peptide surrounding amino acid 180 of human NCS-1
来源宿主
兔
反应性
经检测验证,可与人、大鼠种属来源的NCS-1蛋白发生免疫反应.
保存建议
提供的NCS-1抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA和0.01%硫柳汞的PBS缓冲液中.请将该NCS-1抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.