Intended Use：This SRB cell cytotoxicity BioAssay kit is simple，accurate，reproducible and sensitive. This kit offers an excellent and efficient method for in vitro cytotoxicity studies as well as high-throughput drug screening that can detect between 5000-50,000 cells/well. This assay provides a convenient and non-radioactive alternative to carry out cytotoxicity assays. Relies on the ability of SRB to bind cellular protein components and measure the total biomass.Sample Type：Cell culture： Adherent cells.Range： 5000-50,000 cells/wellSpecificity：MammalianTest Principle：This Sulforhodamine B （SRB） cell cytotoxicity BioAssay is one of the most widely methods used to detect cell viability or drug cytotoxicity. This assay relies on the ability of SRB to bind cellular protein components and measure the total biomass. SRB is a bright-pink aminoxanthene dye that can form an electrostatic complex with basic amino acid residues of proteins in slightly acidic conditions but it can dissociate under basic conditions. It has been widely used for drug toxicity screening against different types of cancerous and non-cancerous cell lines. In addition，this assay is independent of cell metabolic activity and therefore should show less interference by the testing compounds. Since the binding of SRB is stoichiometric，the incorporated dye released from stained cells after washing is directly proportional to the cell biomass and can be measured at 565nm.Kit Components：Fixation Solution，1x55ml20X Washing Solution，1x50ml10X Solubilization Solution，1x22mlSRB Dye Solution，1x50ml20mM Doxorubicin，1x100ulStorage and Stability：Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product，centrifuge the original vial after thawing and prior to removing the cap.
产品特点
针对Sulforhodamine B Cell Cytotoxicity，Colorimetric BioAssay Kit (SRB)该产品的特点优势，欢迎查阅官网提供的产品说明书。
保存建议
建议收到Sulforhodamine B Cell Cytotoxicity，Colorimetric BioAssay Kit （SRB）产品后将其置于-20°C保存。
