The Quick Cell Proliferation BioAssay Kit provides by far the most sensitive and easiest means for quantifying cell proliferation and viability. Simply add the single reagent to cell culture,incubate and read the fluorescence intensity. Intended Use:This fluorescence-based single-step cell viabilty assay utilizes the redox dye (Resazurin) which is not fluorescent,but upon reduction by metabolically active cells,it becomes highly fluorescent (Ex = 530-570 nm; Em = 590 -620 nm). Therefore,viable metabolically active cells can be easily measured.Specificity:MammalianSensitivity:Detect as few as 100 cells.Range:100-100,000 cells/well.Test Principle:The BioAssay utilizes the redox dye resazurin which is not fluorescent,but upon reduction by viable metabolically active cells,the dye becomes highly fluorescent (Ex = 530 - 570 nm; Em = 590-620 nm). Therefore,viable metabolically active cells can be easily measured.Kit Components:Reagent,5ml 500 AssayReagent,25ml 2500 AssayStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product,centrifuge the original vial after thawing and prior to removing the cap.