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细胞分析试剂盒
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中文名称

Protein Synthesis Monitoring BioAssay Kit (Flow Cytometry)

英文名字
Protein Synthesis Monitoring BioAssay Kit(Flow Cytometry)
供应商
USBiological
产品货号
369287
产品报价
¥1/50Tests
产品说明书
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背景资料
USBiological品牌是全球有名的抗原抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理,是行业中少有的致力于服务客户,帮助客户,且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应,专业的产品和解决方案支持,稳健快捷的交货周期,优质放心的售后服务。我们致力于为您提供有价值的产品和服务,在意您的成功!
产品描述
Cells generate a complete set of proteins during division. Protein synthesis is a tightly regulated process and many critical controls in gene expression occur at the level of translation to ensure that production of specific cellular proteins is quickly turned on/off under specific conditions (heat shock,starvation,etc.). Protein synthesis is essential in cell growth,proliferation,signaling,differentiation or death; therefore,the identity and amount of the synthesized proteins are critical parameters in determining the physiological state of the cell. Methods enabling detection and characterization of nascent proteins,or changes in spatial and temporal protein expression/degradation patterns during disease,drug treatments or environmental changes are important tools in assessment of cytotoxicity. Intended Use:Detection of nascent protein synthesisDetection of protein expression and degradation patterns in presence of cytotoxic agents Screening for genotoxic compounds and effectors of protein synthesisSample Type:Suspension or adherent cell culturesSpecificity:MammalianTest Principle:The Protein Synthesis Monitoring BioAssay Kit utilizes a novel and robust chemical method based on an alkyne containing and cell permeable analog of puromycin,O-Propargyl-puromycin (OP-puro). Once inside the cell,OP-puro stops translation by forming covalent conjugates with nascent polypeptide chains. Truncated polypeptides are rapidly turned over by the proteasome and can be detected based on a click reaction with the fluorescent azide. Unlike methionine analogs,OP-puro does not require methionine-free conditions and can be used to label nascent proteins directly in the cell culture. This kit provides sufficient materials for 50 assays to detect nascent proteins synthesized under various physiological conditions,and Cycloheximide,an inhibitor of protein synthesis that serves as an experimental control.Kit Components:Wash Buffer (10X),1x25mlFixative Solution,1x10mlPermeabilization Buffer (10X),1x25mlProtein Label (400X),1x125ulCopper Reagent (500X),1x100ulFluorescent Azide (100X),1x250ulReducing Agent (20X),1x1.25mlCycloheximide (100X),1x100ulStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product,centrifuge the original vial after thawing and prior to removing the cap.
产品特点
针对Protein Synthesis Monitoring BioAssay Kit (Flow Cytometry)该产品的特点优势,欢迎查阅官网提供的产品说明书。
保存建议
建议收到Protein Synthesis Monitoring BioAssay Kit (Flow Cytometry)产品后将其置于-20°C保存。
其他
Usbiological公司是美国著名的抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品,为科研用户提供了诸多超值选择。
注意
该页面的中文产品信息的翻译,仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前,请浏览说明书确认。
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