Quantification of the number of viable cells is an indispensable tool in cell biology research. The MTT Cell Proliferation BioAssay Kit is a sensitive method for quantification of viable cells in proliferation and cytotoxicity assay. The method is based on the conversion of water soluble MTT （3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide） compound to an insoluble formazan product. Viable cells with active metabolism convert MTT into formazan，however，dead cells lose this ability. Thus color formation serves as a useful and convenient marker of only the viable cells. The measured absorbance （590 nm） is proportional to the number of viable cells. This BioAssay kit provides an easy-to-use，non-radioactive，and high-throughput method for cell proliferation，cell viability，chemotaxis，cytotoxicity，and apoptosis.Intended Use：Measurement of cell proliferation in response to growth factors，cytokines，mitogens and nutrients.Analysis of cytotoxic and cytostatic compounds such as anticancer drugs，toxic agents and other pharmaceuticals.Assessment of physiological mediators that inhibit cell growth.Sample Type：Adherent and suspension cellsProliferating and non-proliferating cellsTest Principle：This BioAssay kit provides an easy-to-use，non-radioactive，and high-throughput method for cell proliferation，cell viability，chemotaxis，cytotoxicity，and apoptosis.Kit Components：MTT Reagent，1x50mlMTT Solvent，1x150mlStorage and Stability：Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product，centrifuge the original vial after thawing and prior to removing the cap.
