Intended Use：The assay can be fully automated for high throughput （1 sec/sample） and is extremely sensitive and is ideal for detecting ATP production or consumption in a variety of processes and enzymatic reactions.Sensitivity：The activity profile shows a linear detection range for ATP values as low as 10fmol/assay （1nM）.Specificity：MammalianRange：1nmol-10fmol/assay.Test Principle：Adenosine-5’-triphosphate （ATP） is a central molecule in the chemistry of all living things and is used to monitor many biological processes. An accurate，reliable method to detect minute ATP levels such as the Luciferase/Luciferin system has broad application. Conventional Luciferase/Luciferin ATP detection systems are unstable since luciferase loses activity rapidly. Luciferase; a genetically modified variant derived from the Luciferase of Diaphanes pectinealis （Chinese Firefly） endemic to Yunnan province，China. We designate our recombinant highly stable luciferase rLucHS. Compared to the normal phenotype of Photinus pyralis，rLucHS provides enhanced stability，excellent sensitivity，and a broader and more physiologically relevant effective pH range. At all pH’s below ~ 8.2，rLucHS has a significantly higher relative activity than Photinus luciferase and is stable for weeks at room temperature and >60 minutes at 37°C. Using the protocol outlined here，the quantitation range is between approximately 1nmol-10fmol/assay. The specific activity of rLucHS is ~ 5x10e11RLU/mg protein.Kit Components：Reaction Buffer，2ml/100 AssaysReaction Buffer，20ml/1000 AssaysReconstitution Buffer，1.1ml/100 AssaysReconstitution Buffer，11ml/1000 AssaysEnzyme Mix （lyophilized），1xvial 100/1000 assaysATP Standard （MW 551） （lyophilized），1xvial 100/1000 AssaysStorage and Stability：Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product，centrifuge the original vial after thawing and prior to removing the cap.
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保存建议
建议收到Highly Stable ATP Bioluminescence BioAssay Kit产品后将其置于-20°C保存。
