The Cell Proliferation BioAssay Kit （Fluorometric） provides an easy and quantitative method to measure cell proliferation and cytotoxicity. The assay is based on a nuclear dye that specifically binds to nucleic acid in the cell and generates green fluorescence. The generated fluorescent intensity is directly proportional to the cell number，which can be quantified by measuring fluorescence （Ex/Em = 480/538 nm）.Intended Use：Measurement of cell proliferation in response to growth factors，cytokines，mitogens，and nutrientsAnalysis of cytotoxic and cytostatic compounds such as anticancer drugs，toxic agents，and other pharmaceuticalsAssessment of physiological mediators and antibodies that inhibit cell growthRange：25-60,000 cellsSample Type：Adherent or suspension cellsTest Principle：This BioAssay kit provides a just add-and-read，non-radioactive，and high-throughput method for cell proliferation and apoptosis. The assay is rapid and more sensitive than MTT，XTT，or MTS-based assays and can detect a wide linear range of 25-60,000 cells.Kit Components：Cell Lysis Buffer （5X），1x25mlNuclear Dye （200X），1x0.65mlStorage and Stability：Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product，centrifuge the original vial after thawing and prior to removing the cap.
