Intended Use：Measurement of cell proliferation in response to growth factors，cytokines，mitogens and nutrientsAnalysis of cytotoxic and cytostatic compounds such as anti-cancer drugs，toxic agents and other pharmaceuticalsAssessment of physiological mediators and antibodies that affect cell growth.Sample Type：Adherent and suspension cellsProliferating and non-proliferating cellsSensitivity：As low as 50 viable cells in less than 30 minTest Principle：This BioAssay kit provides an easy- to-use，non-radioactive，and high-throughput method for cell proliferation，cell viability，chemotaxis，cytotoxicity and apoptosis.Quantification of number of viable cells is an indispensible tool in Cell Biology research. The Calcein AM Cell Viability BioAssay Kit is a fluorometric method for extremely sensitive quantification of viable cells that can detect as low as 50 viable cells in less than 30 min. Calcein AM is a non-fluorescent，hydrophobic compound that easily penetrates intact and live cells. Hydrolysis of Calcein AM by intracellular esterase produces a hydrophilic，strongly fluorescent compound that is retained in the cell cytoplasm and can be measured at Ex/Em = 485/530nm. The measured fluorescence intensity is proportional to the number of viable cells.Kit Components：Calcein AM，2x1vialCalcein Dilution Buffer，1x100mlStorage and Stability：Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product，centrifuge the original vial after thawing and prior to removing the cap.
产品特点
针对Calcein AM Cell Viability BioAssay Kit (Fluorometric)该产品的特点优势，欢迎查阅官网提供的产品说明书。
保存建议
建议收到Calcein AM Cell Viability BioAssay Kit （Fluorometric）产品后将其置于-20°C保存。
