Mammalian forkhead members of the class O （FOXO） transcription factors，including FOXO1，FOXO3a，and FOXO4，are implicated in the regulation of a variety of cellular processes，including the cell cycle，apoptosis，DNA repair，stress resistance，and metabolism. FOXO proteins are negatively regulated by the phosphatidylinositol 3-kinase-Akt signaling pathway，which is activated by growth factors and cytokines. Recent studies indicate that the activities of FOXO proteins are also regulated by oxidative stress，which induces their phosphorylation，translocation to the nucleus，and acetylation-deacetylation. Similar to the tumor suppressor p53，FOXO is activated by stress and induces the expression of genes that contribute to cell-cycle arrest，suggesting that it also functions as a tumor suppressor.
应用类型
Western blotting （0.5-4 ug/ml）. Jurkat cell lysate （Cat.# 2401-100） and rat liver or rat kidney tissue lysate can be used as a positive control. A ~75 kDa band should be detected.
免疫原
Synthetic peptide corresponding to residues on the C-terminus of human FOXO3a
来源宿主
兔
反应性
经检测验证，可与人、小鼠种属来源的FOXO3a蛋白发生免疫反应.
保存建议
提供的FOXO3a抗体产品为100 ug 无色溶液，其浓度为0.2 mg/ml，溶解于包含有0.5% BSA， 30%甘油和0.01%硫柳汞的PBS缓冲液中.请将该FOXO3a抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
