Noxa is a BH3-only member of the Bcl-2 family. Expression of Noxa gene involves direct activation of its promotor by p53. Increased expression of Noxa protein occurs in normal thymocytes but not in p53-deficient thymocytes. Coimmunoprecipitation data suggest that Noxa protein may interact with proteins belonging to the Bcl-2 family，such as Bcl-xL and Mcl-1. Blocking the endogenous Noxa induction results in the suppression of apoptosis. These suggest Noxa may represent a candidate mediator of p53-induced apoptosis.
应用类型
Western blotting （0.5-4 ug/ml）. However， the optimal concentrations should be determined individually. The antibody recognizes mainly ~16 kDa band in human， mouse and rat samples. A ~34 kDa band is also detected to the lesser extent. Reactivity to other species has not been tested.
免疫原
Synthetic peptide surrounding amino acid 100 of human Noxa
来源宿主
兔
反应性
经检测验证，可与人、小鼠、大鼠种属来源的Noxa蛋白发生免疫反应.
保存建议
提供的Noxa抗体产品为100 ug 无色溶液，其浓度为0.2 mg/ml，溶解于包含有0.5% BSA， 30%甘油和0.01%硫柳汞的PBS缓冲液中.请将该Noxa抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
