Beclin is a Bcl-2 binding protein. Its structure includes a Bcl-2 binding coiled-coil region，and a leucine-rich nuclear export signal （NES）. Beclin protein colocalizes with intracytoplasmic organelles and nuclei in normal COS7 and MCF7 cells. Beclin interaction with Bcl-2 may be involved with host viral defense，since over expression of beclin inhibits Sindbis virus replication and expression of beclin lacking the Bcl-2 binding domain has no antiviral effects.
应用类型
Western blotting （0.5-2 ug/ml）. However， the optimal concentrations should be determined individually. Other applications have not been tested. The antibody recognizes ~53 kDa beclin 1 in Jurkat cell lysate. A weak band of ~110 kDa （possibly dimer） can also be observed in Jurkat cells. In mouse intestine tissue lysate， the antibody recognizes a ~100 kDa band （possibly dimer）. Reactivity to other species has not been tested.
免疫原
Synthetic peptide corresponding to residues around amino acid 25 of mouse Beclin 1
来源宿主
兔
反应性
经检测验证，可与人、小鼠种属来源的Beclin 1蛋白发生免疫反应.
保存建议
提供的Beclin 1抗体产品为100 ug 无色溶液，其浓度为0.2 mg/ml，溶解于包含有1% BSA， 50%甘油和0.02%硫柳汞的PBS缓冲液中.请将该Beclin 1抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
