The Bovine Serum Albumin （BSA） BioAssay ELISA Kit is a competitive inhibition immunoassay for the in vitro quantitative measurement of BSA in plasma，serum，tissue homogenates，cell lysates，cell culture supernatants and other biological fluids.Specificity：GeneralDetection Range：246.9 - 20,000ng/mlSensitivity：<87.2ng/mlPrecision：Intra-Assay CV： <10%Inter-Assay CV： <12%Test Principle：This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific for BSA has been pre-coated onto a microplate. A competitive inhibition reaction occurs between biotin-labeled BSA and unlabeled BSA （standards or samples） for limited binding sites on the pre-coated antibody. After incubation，unbound materials are washed off. Next，avidin conjugated to horseradish peroxidase （HRP） is added to each microplate well and incubated. The amount of bound HRP conjugate is inversely proportional to the concentration of BSA in the sample. After addition of the substrate solution，the intensity of color developed is inversely proportional to the concentration of BSA in the sample.Kit Components：*365783A： Microtiter Plate，96 wells，Pre-coated，ready to use.*365783B： Standard，2x1vial 365783C： Standard Diluent，1x20ml*365783D： Detection Reagent A，1x120ul*365783E： Detection Reagent B，1x120ul 365783F： Assay Diluent A，1x12ml365783G： Assay Diluent B，1x12ml365783H： TMB Substrate，1x9ml365783K： Stop Solution，1x6ml365783L： Wash Buffer，30X，1x20mlStorage and Stability：Store *365783A，*365783B，*365783D and *365783E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened，it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product，centrifuge the original vials after thawing and prior to removing the cap.Assay Procedure Summary：1. Prepare all reagents，samples and standards.2. Add 50ul standard or sample to each well，then add 50ul Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C.3. Aspirate and wash 3 times.4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.5. Aspirate and wash 5 times.6. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37°C.7. Add 50ul Stop Solution. Read absorbance at 450nm immediately.
产品特点
针对Bovine Serum Albumin (BSA) BioAssay ELISA Kit (General) (High Sensitivity)该产品的特点优势，欢迎查阅官网提供的产品说明书。
保存建议
建议收到Bovine Serum Albumin （BSA） BioAssay ELISA Kit （General） （High Sensitivity）产品后将其置于4°C至-20°C保存。
