Vimentin,a intermediate filament protein,is a general marker of cells originating in the mesenchyme. Vimentin is frequently co-expressed with other members of the intermediate filament family such as the cytokeratins,in neoplasm.
应用类型
Western blotting (0.5-4 ug/ml), immunoprecipitation (10-20 ug/ml) and Immunohistochemistry (10-20 ug/ml). However, the optimal concentrations should be determined individually.
免疫原
Synthetic peptide surrounding amino acid 448 of human Vimentin
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠、牛、鸡种属来源的Vimentin蛋白发生免疫反应.
保存建议
提供的Vimentin抗体产品为100 ug 无色溶液,其浓度为0.2 mg/ml,溶解于包含有30%甘油, 0.5% BSA和0.01%硫柳汞的PBS缓冲液中.请将该Vimentin抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.