- 中文名称
VEGF BioAssay ELISA Kit (Human) (Vascular endothelial growth factor，VEGF，Vascular permeability factor，VPF)
- 英文名字
- VEGF BioAssay ELISA Kit(Human)(Vascular endothelial growth factor，VEGF，Vascular permeability factor，VPF)
- 供应商
- USBiological
- 产品货号
- 361426
- 产品报价
- ￥1/96Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The gene for human VEGF is organized into 8 exons. As a result of alternative splicing，at least 4 transcripts encoding mature monomeric VEGF containing 121，165，189，and 206aa residues （VEGF121，VEGF165，VEGF189，and VEGF206）. VEGF is a highly conserved protein that has cross-species activity. Between human，rat or bovine VEGF，84-94% sequence identity has been observed. In vitro，VEGF is a potent endothelial cell mitogen. In cultured endothelial cells，VEGF can activate phospholipase C and induce rapid increases of free cytosolic Ca2+. VEGF has also been shown to be chemotactic for monocytes and osteoblasts. Based on its in vitro and in vivo properties，VEGF is expected to play important roles in inflammation and during normal and pathological angiogenesis，a process that is associated with wound healing，embryonic development，and growth and metastasis of solid tumors.Sample Type：Cell culture supernatants，serum，plasma，and tissueIntended Use：The Human VEGF BioAssay ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of Human VEGF concentrations in cell culture supernatants，serum，plasma，and tissue.Sensitivity：4pg/mlRange：15.625-1000pg/mlSpecificity：Recognizes human VEGF. There was no detectable cross reactivity with any of the tested proteins following IL-1,2,3,4,5,6，8,10,12,TNF,TGF and IFN.Sample Volume：100ul/wellTest Principle：This assay employs the Sandwich immunoassay technique. An anti-h VEGF monoclonal coating antibody is adsorbed onto microwells. VEGF present in the sample or standard binds to antibodies adsorbed to the microwells. Following incubation unbound sample or standard are removed during a wash step. a Biotinylated anti-h VEGF antibody is added and binds to VEGF captured by the first antibody. Following incubation unbound Biotinylated anti-h VEGF antibody is removed during a wash step. A Streptavidin-HRP is added and binds to Biotinylated anti-h VEGF antibody. Following incubation unbound Streptavidin-HRP is removed during a wash step. A colored product is formed in proportion to the amount of VEGF present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven VEGF standard dilutions and VEGF sample concentration determined.Kit Components：Aluminium pouches with a Microwell Plate： 8x12Standard： 2xVialBiotinylated antibody： 2xVialStreptavidin-HRP： 2xVialAssay Buffer （25ml/Vial）： 2xVialWash Buffer Concentrate 20x （30ml/Vial）： 1xVialTMB Substrate Solution： 1x12mlStop Solution （12ml/Vial）： 1xVialPlate Covers- Adhesive strips： 3xstripsInstruction： 1xStorage and Stability：Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product，centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对VEGF BioAssay ELISA Kit (Human) (Vascular endothelial growth factor，VEGF，Vascular permeability factor，VPF)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到VEGF BioAssay ELISA Kit （Human） （Vascular endothelial growth factor，VEGF，Vascular permeability factor，VPF）产品后将其置于4°C至-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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