Human VCAM-1 （CD106），a member of the immunoglobulin superfamily，is a cell surface protein expressed by activated endothelial cells and certain leukocytes such as macrophages. VCAM-1 expression is induced by IL-1beta，IL-4，TNF-alpha and IFN-gamma. VCAM-1 binds to leukocyte integrins VLA-4 and Integrin alpha 4 beta 7.Sample Type：Cell culture supernatants，serum，plasma，and tissueIntended Use：The Human VCAM-1 BioAssay ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of Human VCAM-1 concentrations in cell culture supernatants，serum，plasma，and tissue.Sensitivity：60pg/mlRange：125-8000pg/mlSpecificity：Recognizes human VCAM-1.Sample Volume：100ul/wellTest Principle：This assay employs the Sandwich immunoassay technique. An anti-h VCAM-1 monoclonal coating antibody is adsorbed onto microwells. VCAM-1 present in the sample or standard binds to antibodies adsorbed to the microwells. Following incubation unbound sample or standard are removed during a wash step. a Biotinylated anti-h VCAM-1 antibody is added and binds to VCAM-1 captured by the first antibody. Following incubation unbound Biotinylated anti-h VCAM-1 antibody is removed during a wash step. A Streptavidin-HRP is added and binds to Biotinylated anti-h VCAM-1 antibody. Following incubation unbound Streptavidin-HRP is removed during a wash step. A colored product is formed in proportion to the amount of VCAM-1 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven VCAM-1 standard dilutions and VCAM-1 sample concentration determined.Kit Components：Aluminium pouches with a Microwell Plate： 8x12Standard： 2xVialBiotinylated antibody： 2xVialStreptavidin-HRP： 2xVialAssay Buffer （25ml/Vial）： 2xVialWash Buffer Concentrate 20x （30ml/Vial）： 1xVialTMB Substrate Solution： 1x12mlStop Solution （12ml/Vial）： 1xVialPlate Covers- Adhesive strips： 3xstripsInstruction： 1xStorage and Stability：Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product，centrifuge the original vial after thawing and prior to removing the cap.
