Intercellular adhesion molecule 1 （ICAM-1），also known as CD54，binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes，epithelial and resting endothelial cells，as well as some other cell types，but expression can be stimulated by IFN-gamma，TNF-alpha，IL-1 beta and LPS. Soluble ICAM-1 is found in a biologically active form in serum，probably as a result of proteolytic cleavage from the cell surface.Sample Type：Cell culture supernatants，serum，plasma，and tissueIntended Use：The Human sICAM-1 BioAssay ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of Human sICAM-1 concentrations in cell culture supernatants，serum，plasma，and tissue.Sensitivity：60pg/mlRange：125-8000pg/mlSpecificity：Recognizes human sICAM-1.Sample Volume：100ul/wellTest Principle：This assay employs the Sandwich immunoassay technique. An anti-h sICAM-1 monoclonal coating antibody is adsorbed onto microwells. sICAM-1 present in the sample or standard binds to antibodies adsorbed to the microwells. Following incubation unbound sample or standard are removed during a wash step. a Biotinylated anti-h sICAM-1 antibody is added and binds to sICAM-1 captured by the first antibody. Following incubation unbound Biotinylated anti-h sICAM-1 antibody is removed during a wash step. A Streptavidin-HRP is added and binds to Biotinylated anti-h sICAM-1 antibody. Following incubation unbound Streptavidin-HRP is removed during a wash step. A colored product is formed in proportion to the amount of sICAM-1 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven sICAM-1 standard dilutions and sICAM-1 sample concentration determined.Kit Components：Aluminium pouches with a Microwell Plate： 8x12Standard： 2xVialBiotinylated antibody： 2xVialStreptavidin-HRP： 2xVialAssay Buffer （25ml/Vial）： 2xVialWash Buffer Concentrate 20x （30ml/Vial）： 1xVialTMB Substrate Solution： 1x12mlStop Solution （12ml/Vial）： 1xVialPlate Covers- Adhesive strips： 3xstripsInstruction： 1xStorage and Stability：Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product，centrifuge the original vial after thawing and prior to removing the cap.
