HDAC family members have been divided into two classes. Class I of the HDACs comprises four members,HDAC-1,2,3,and 8,each of which contains a deacetylase domain exhibiting from 45 to 93% identity in amino acid sequence. Class II of the HDACs comprises HDAC-4,5,6,and 7,the molecular weights of which are all about two-fold larger than those of the class I members,and the deacetylase domains are present within the C-terminal regions,except that HDAC-6 contains two copies of the domain,one within each of the N-terminal and C-terminal regions. Human HDAC-1,2 and 3 were expressed in various tissues,but the others (HDAC-4,5,6,and 7) showed tissue-specific expression patterns.
应用类型
Western blotting (0.5-4 ug/ml), Immunoprecipitation and immunofluorescence. However, the optimal concentrations should be determined individually. The antibody recognizes a ~110-140 kDa of HDAC-9. A 75 kDa and a 60 kDa bands can also be detected in some tissue lysates.
免疫原
Synthetic peptide surrounding amino acid 25 of human HDAC-9
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠种属来源的HDAC9蛋白发生免疫反应.
保存建议
提供的HDAC9抗体产品为100 ug 无色溶液,其浓度为0.2 mg/ml,溶解于包含有30%甘油, 0.5% BSA和0.01%硫柳汞的PBS缓冲液中.请将该HDAC9抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.