MCP-1，also known as monocyte chemotactic and activating factor （MCAF），was initially purified based on its ability to chemoattract monocytes. Subsequent to its cloning and sequencing，it became evident that this protein is also identical to the product of the human JE gene. The JE gene，originally identified in mouse fibroblasts，is a platelet-derived growth factor （PDGF）-inducible gene.Sample Type：Cell culture supernatants，serum，plasma，and tissueIntended Use：The Mouse MCP-1 BioAssay ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of Mouse MCP-1 concentrations in cell culture supernatants，serum，plasma，and tissue.Sensitivity：2pg/mlRange：250-3.9pg/mlSpecificity：Recognizes mouse MCP-1.Sample Volume：100ul/wellTest Principle：This assay employs the Sandwich immunoassay technique. A MCP-1 monoclonal coating antibody is adsorbed onto microwells. MCP-1 present in the sample or standard binds to antibodies adsorbed to the microwells. Following incubation unbound sample or standard are removed during a wash step. a Biotinylated anti-m MCP-1 antibody is added and binds to MCP-1 captured by the first antibody. Following incubation unbound Biotinylated anti-m MCP-1 antibody is removed during a wash step. A Streptavidin-HRP is added and binds to Biotinylated anti-m MCP-1 antibody. Following incubation unbound Streptavidin-HRP is removed during a wash step. A colored product is formed in proportion to the amount of MCP-1 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven MCP-1 standard dilutions and MCP-1 sample concentration determined.Kit Components：Aluminium pouches with a Microwell Plate： 8x12Standard： 2xVialBiotinylated antibody： 2xVialStreptavidin-HRP： 2xVialAssay Buffer （25ml/Vial）： 2xVialWash Buffer Concentrate 20x （30ml/Vial）： 1xVialTMB Substrate Solution： 1x12mlStop Solution （12ml/Vial）： 1xVialPlate Covers- Adhesive strips： 3xstripsInstruction： 1xStorage and Stability：Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product，centrifuge the original vial after thawing and prior to removing the cap.
产品特点
针对MCP-1 BioAssay ELISA Kit (Mouse) (C-C motif chemokine 2，HC11，Monocyte chemoattractant protein 1，Monocyte chemotactic and activating factor，MCAF，Monocyte chemotactic protein 1，MCP-1，Monocyte secretory protein JE，Small-inducible cytokine A2，C该产品的特点优势，欢迎查阅官网提供的产品说明书。
保存建议
建议收到MCP-1 BioAssay ELISA Kit （Mouse） （C-C motif chemokine 2，HC11，Monocyte chemoattractant protein 1，Monocyte chemotactic and activating factor，MCAF，Monocyte chemotactic protein 1，MCP-1，Monocyte secretory protein JE，Small-inducible cytokine A2，CCL2，MCP1，SCYA2）产品后将其置于4°C至-20°C保存。
